Q. How is micropropagation carried out?
A small piece of plant tissue (explant) is taken from the donor plant and cultured on a nutrient medium in sterile containers. By altering the composition of the medium and the environmental conditions (temperature, light regime, etc.) the development of this piece of tissue can be directed along different patterns and finally the whole plant can be regenerated. The offspring all come from a single plant and thus have identical genetic make-ups to each other and to the mother plant. They are thus called clones.
Q. What are the advantages of micropropagation over the conventional method of growing plants?
It is a fast method of propagation, producing thousands of plantlets in
a matter of months.
Q. What are the constituents of tissue culture nutrient media?
Q. How can we ensure that the plants produced from tissue culture are free from viruses?
This can be achieved by developing a biophysical, immunological and molecular-technique based virus-diagnosis programme, and molecular-based quality control programme for the plant species of interest. Immunodiagnosis is the most useful technique using polyclonal and monoclonal antibodies raised against viruses. Polyclonal antibodies are now available in the country for groups of viruses such as potyviruses, tospoviruses, tobamoviruses, potexviruses, luteoviruses, badnaviruses, closteroviruses, etc. These polyclonal antibodies have been very useful in the identification of specific viruses and their strains, and for plant virus diagnosis.
Q. How do plant growth regulators affect plant morphogenesis in culture?
Two plant growth regulators affect plant differentiation:
Auxins: Stimulate root development
Organogenesis: The process of initiation and development of a structure that shows natural organ form and/or function.
Q. What is embryogenesis?
Embryogenesis: The process of initiation and development of embryos or embryo-like structures from somatic cells (somatic embryogenesis).
are the various stages of micropropagation?
Stage I - Initiation of culture
Stage II - Multiplication
Stage III - Rooting
Stage IV - Transfer to soil
Q. What is the embryo